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Macrophage activation syndrome

Am J Physiol Cell Physiol 2020;320:C375C91

10.1182/blood-2007-04-081703 Blood 78 NasimuzzamanM.MalikP

Ni-INDIGO beads (20 l) (Cube Biotech, 75105) were added and excess peptide was removed using a PD MidiTRAP G-25 (Cytiva) followed by size-exclusion chromatography (Superdex 75) in 25 mM HEPES, 150 mM NaCl and 1 mM TCEP, pH 7.5